Objective:To develop a HPLC method, using diazepam as internal standard,to determine nisoldipine content and content uniformity
in its tablets Method:The chromatographic conditions included C 18 analytical column,the mobile phase consisted of methanol-H 2O(65∶35), and UV detector at 237 nm Results:Under this condition,there was a good linear relationship in the concentration range of 8～80 μg·mL -1 , the average recovery was 99 9%, and the relative standard deviation was 0 87% Conclusions:The proposed method is selective,simple and accurate,the decomposed product can be separated as well Therefore,the quality of nisoldipine tablets can be effectively controlled Key words HPLC,nisoldipine, tabletsats$$$$ Wen Chun he,SU Dong mei,WANG Xi ai (Henan Provincial Institute for Prevention and Treatment of Occupational Diseases,Zhengzhou,Henan 450052,China) Abstract:Objective To explore the influence of germanium 132( 132 Ge)on serum activity of
superoxide dismutase(SOD),level of lipid peroxidation(LPO)and function of liver Kupffer cells in rats with silicosis.Methods Experimental rats were divided into three groups.Groups A and B were instilled intratracheally with suspension of mixed quartz dust,and Group C with normal saline.Group A was fed with water containing 132 Ge and Groups B and C were fed with tap water.After six month,blood specimens were collected from animal hearts.Serum activity of SOD iso enzymes was determined with nitrite formation method,serum LPO level was determined with thio barbiturate colorimetry,and cellular immunity of silicotic rats was determined by tumor necrosing factors(TNF)test.Results Serum total activity of SOD increased more significantly in Group A than in Group B(P<0 01).Serum LPO level in Group A(6 17±1 27)decreased significantly than in Group B(8 31±1 11).Ability to induce TNF in liver Kupffer cells reduced slightly in Group B and increased slightly in Group A.Conclusion 132 Ge can play certain roles in decrease in damage to macrophages and improvement of immune function in rats.