Objective \ To investigate the effect of Kadsurenone on the implantation of
mouse embryos and to observe the
cytochemical
and ultra structural changes.\ Methods \ On
the day 2 of pregnancy, Kadsurenone in different dosages was intrauterohornly injected into
the mice. On the day 8 and day 10 of pregnancy, the expression of WGA and ConA receptors as
well as ultrastructural changes in
trophoblastic cells and decidual cells were observed.\
Results \ Cytochemical observations showed that the positive reaction of WGA and ConA
receptors of trophoblastic cells weakened along with the increasing dosages of Kadsurenone,
notablly with the WGA staining. Ultrastructurally, the cytoplasms of trophoplastic cells within
the superficial area in the experimental groups became less dense, nuclei pyknosed; while in
the deeper area, the lysosomes in the trophoblastic cells remarkablly increased in number,
Even the myelinated bodies were found in these cells. In larger decidual cells, the lysosomes
were also greatly increased accompanied by vacuolation of the cytoplasm and rupture of the
nuclear membrane.\ Conclusions \ By means of decreasing the amount of glycoprotein in WGA
and ConA receptors in trophoblastic cells, Kadsurenone can interfere with the intercellular
adherence and attachment, destroy the enviroment where the embryos survived and developed
and inhibit the process of
implantation.