AIM To observe the protective effect of Qinling chongji (QL) on liver and renal functions, and the effect on blood biochemistry
in DHBV induced
hepatitis nestling ducks. METHODS When positive serum DHBV was detected in seven day infected nestling ducks, animals were randomly divided into five groups: ①normal saline (NS); ②positive control drug, ACV 100*!mg·kg -1 . ③ QL 10*!g·kg -1 (QLb); ④QL 5g·kg -1 (QLm);⑤ QL 2 5*!g·kg -1 (QLs). Five to six animals were used in each group. Drugs were administered orally, twice daily for 10 days. The blood samples were taken from leg vein 10 days after treatment and sera were separated at once. RESULTS Blood biochemical indexes of nestling ducks showed significant abnormality 7 days after DHBV infection, of which liver function (ALT, AST, SB)and renal function (UN, CR) changed the most, secondly,LDH level increased significantly. The animals were treated with different doses of QL for 10 days, above normal biochemical indexes were lowered in QLb (10*!g·kg -1 ) group ( P <0 05 , P <0 01 ). Liver pathological examination demonstrated that the constructions of liver leaflet in DHBV control group were damaged completely, while those in QLb group maintained intact. CONCLUSION QL can protect liver tissues in DHBV induced hepatitis nestling duck and could decrease the serum levels of ALT, AST, SB, UN and CR, especially the later two. The liver and renal functions were improved after treatment with QL, so it is worthwhile to perform further clinical studies on this anti hepatitis drug.