EFFECT OF CEFODIZIME ON CYTOKINE ACTIVITY AND THE EXPRESSION OF CYTOKINE mRNA

ABSTRACT Objective: To investigate the effects of cefodizime (CDZM) on production of TNFα, IL-6 and their mRNA expression in murine peritoneal macrophage (PM) from normal and Staphylococcus aureus infected mouse. Methods: Mouse PM were treated in vitro with cefodizime, cell culture supernatant was separated. TNFα was detected by biological assay, IL6 by ELISA, and the level of mRNA with the method of reverse transcriptionpolymerase chain reaction (RTPCR). Results:TNFα production was decreased in the supernatant of normal murine PM while increased in the supernatant of Staphylococcus aureus infected murine PM by the effect of CDZM. The maximal effect was observed after 48 h of incubation. CDZM significantly restrained TNFα mRNA expression in normal murine PM at dose of 200 mg/L. Its promoting effect to TNFα mRNA expression had no statistical difference to the basal level.　CDZM slightly inhibited the production of IL-6 in the supernatant of normal murine PM after 48 h incubation, but significantly stimulated the production of IL-6 in Staphylococcus aureus infected murine PM. Coordinate result was obtained in the expression of IL-6 mRNA. Conclusion: The effects of CDZM on TNFα and IL-6 production are different between normal and Staphylococcus aureus infected mouse.The corresponding mRNA expression are coordinately different.