Using nerve cells from chicken embryo,the effects of
allyl chloride on
intracellular Ca 2+ , Ca 2+ free
calmodulin(CaM) , cyclic AMP(cAMP) contents and Ca 2+ /CaM dependent protein kinase Ⅱ (Ca 2+ /CaM PK Ⅱ) activity were studied. The results showed that as allyl chloride at the concentrations of 1.02 and 2.04 mmol·L 1 added, the contents of Ca 2+ were increased by 4.2 and 6.2 times, cAMP was lifted 22% and 39%, and the activity of Ca 2+ /CaM PK Ⅱ was raised over 2.8 and 5.0 times, compared with the control, respectively(P<0.01). However, the content of Ca 2+ free CaM was markedly decreased by 55% and 69% (P<0.01). The results suggest that the allyl chloride induced accumulations of microtubule and neurofilament in pathological nerve axon may be related to the
intracellular Ca 2+ increase, that increased Ca 2+ in cells activates CaM and Ca 2+ /CaM PK Ⅱ, and then induces the microtubule associated protein 2 and tau protein phosphorylation, and eventually inhibits the assembly of microtubule and results in the accumulation of the disassembled microtubule in the nerve axon.