AIM: TO clarify the role of
DL111-IT when combined with
mifepristone (Mif) on
termination of early pregnancy. METHODS: Progesterone receptors (PR) was measured using radioligand assay ( RA ), and peroxidase-antiperoxidase ( PAP ) immuno-histochemistry. Decidual cells (DC) were estimated using cell culture and histological examination (HE) (including fetus). RESULTS: DL111-1T 100 mg · kg-1 im, Mif 5 mg·kg-1 ig, DL111-IT 16 mg·kg-1 im ± Mif 1.5 mg·kg-1 ig and tea seed oil (TSO) 2 mL·kg-1 im on d 7 of pregnancy in rats, DC and
fetus of treated
groups were found to be degenerated at 24 h after treatment, at 48 h after treatment, PAP labeling index (%) of uterus PR of 4 groups were 22 ± 4, 18.7 ± 2.9, 10.3 ±1.2, 52 ± 15, respectively. Rats im DL111-IT 100 mg·kg-1 24 h after treatment, the quantity of PR decreased by 47 % vs that of ISO. The affinity of PR with Mif and progesterone did not change. Cultured human DC were exposed to DL111-IT and Mif 0 -50 mg·L-1, alone or combinatively, for 24 h. LD50(mg·L-1) were: DL111-IT 18.1 (15.1-21.4), Mif 25.0 (23.1 - 26.9), DL111-IT5.0 plus Mif 3.5 (1.8-6.5) or Mif 5.0 plus DL111-IT 4.6 (1.1-7.3), respectively. CONCLUSION: DL111-IT enhanced action of Mif on DC, reduced quantity of PR, so the 2 drugs had the synergistic action in termination of early pregnancy.
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