Aim:To evaluate the antimotion sickness action of transdermally administered scopo- lamine ointment,Methods:Two experimental models of nystagmus in rabbits were induced by horizon-tal rotation and by surgical destruction of one-side labyrinth.Electronystagmograms were recorded inthese models before and after transdermal administration of scopolamine ointment with three differentdoses(0.19 mg/9cm2,0.32 mg/16 cm2 and 0.51 mg/25 cm2)or a piece of scopolamine plaster(0.32mg),and the effects of scopolamine ointment or plaster on nystagmic parameters(slow-phase velocity,frequency, and duration) were observed. Results:Transdermal administration of scopolamine ointmentsignificantly inhibited both slow-phase velocity of nystagmus induced by horizontal rotation and slow- phase velocity of spontaneous nystagmus caused by surgical destruction of left-side labyrinth. The inhi- bition occurred at 15 minutes after administration with doses of 0.51 mg/25 cm2 and 0. 32 mg/16 cm2 and maintained for over 4 hours with dose of 0. 51 mg/25 cm2. There was an obvious dose-effect rela-tionship among three different doses of scopolamine ointment. The onset of nystagmic inhibiting effectof scopolamine ointment was faster than that of scopolamine plaster.Conclusion:Scopolamine ointmentpossesses better antimotion sickness effect than plaster.