AIM:To study the effects of propylene glycol mannate sulfate (PGMS) on platelet adhesion,
aggregation and
thrombosis in abdominal sorta in rabbits. METHODS: Platelet adhesion assay was performed with a platelet-adhesion-meter. The platelet concentration was determined with an electronic particle counter, and the total radio-activity was determined wiht Clinigamma-1272. RESULTS: PGMS inhibited washed
platelet aggregation induced by thrombin in vitro. The value of IC50 was 0.9 mg·L-1. (95 % confidence limit = 4. 1-13.7 mg·L-1). At 10, 30, and 60 min after iv PGMS 75 mg·kg-1, the
inhibition rates of platelet adhesion were 90. 4 %, 41. 8 %, and 26. 3 % and the inhibition rates of platelet aggregation induced by thrombinwere 99 % , 122 % , and 110 % , respectively. It did not exhibit any noticeable effect on platelet aggregation induced by ADP and collagen at this dosage. After 1. 5 h of iv PGMS 75 mg . kg-1 and total autologous 111 In-platelets 3. 3×109 the
radioactivity and dry weight of the injured and uninjured segments were determined. The PGMS group showed a significantly lower radioactivity (1.1±0. 6 MBq), 111 In-platelets (8-3±s 3. 3 ×108) as welll as the radioactivity deposited.g-1 out of the total radioactivity infused % (0. 24+ 0. 21) compared with the control group (3. 7 ±0. 5 MBq, 24. 6 ±3. 5 ×108, and 0. 86±0.25, respectively). CONCLUSION:PGMS prevented the platelet adhesion and aggregation at the injured arterial wall.