Bencynonate is a newly
developed anticholinergic drug.After a dose( 2 mg/per- son or
4mg/person)of bencynonate, the circulating concentrations are so low
that therapeutic drug monitoring studies have prcviously been
impossible. Radioisotope assay and radioaccepter assay had been used
in rat experlments, but the detection limits of these methods Were
not sensitive enough for the therapeutic drug monitoring. Further
,the two methods were unsuitable in the human,For evalua- tion of the
pharmacokinetics and the
relative bioavailability of
Bencynonate in
man,a gas chromatographic─mass spectromectric
method for
quantitative analysis of bencynonate in human
plasma was developed.
Deuterdted bencynonate served as the internal
standard and
selected-ionmonitoring the fragments of bencynonate and internal
standard permitted the quantitation ofbencynonate down to 25
pg·ml-1 of plasma. The linearity was in the range from 25 pg·ml-1
to 3 ng· ml-1 of bencynonate plasma concentration. At 0.25 ng·
ml-1 level the recovery and relative stan-dard deviation of variation
are 54.3%and 19.1%, respectively. Application of the method to
clinic-al studies gave the results of pharmacokinctics and relative
bioavailability of bencynonate in man.
More abstracts about the QUANTITATIVE ANALYSIS OF BENCYNONATE IN HUMAN PLASMA USING
ADEUTERATED INTEKNAL STANDARD BY GC