Objective The aim of the present study was designed to explore the effect of(±)-3-n-butylph-thalide(NBP)on ATPase,anti-oxidant enzymes
activities and lipid peroxidation of mitochondria and cerebral
cortex in rats subjected to24hours of reperfusion following2hours of cerebral ischemia(tMCAO).Methods Activities of
SOD(Superoxide Dismutase),GSH-Px(glutathione Peroxidase,)and CAT(Catalase),and MDA level of mitochondria or cortex were measured by using biochemical met-hods in tMCAO rats.Results(1)The activities of
mitochondrial Na + K + -ATPase,Ca 2+ -ATPase and Mg 2+ ATPase were found to decrese significantly in the
vehicle group(ishemia+saline).Pre-treatment with NBP(5,10,20mg /kg,ip)10min before tMCAO markedly enhanced the activities of Na + K+-ATPase and Ca 2+ -ATPase,compared with vehicle group.(2)The activities of SOD and mitochondrial GSH-Px were decreased and MDA level increased in vehicle groups as compared with that in sham group(non is chemia+saline).NBP(20mg /kg,ip)significantly enhanced total mitochondrial SOD activity,and also enhanced cerebral cortex total SOD activity(in5,10,20mg /kg groups).However,it had no obvious effect on CuZn-SOD activity.NBP(20mg /kg ip)markedly increased mitochondrial(but not in cerebral cortex)GSH-Px activity;NBP10,20mg /kg markedly decreased mitochondrial MDA level compared with that in vehicle group (P<0.05=.(3)The action of raceme NBP on the increase of the activities of ATPase and antioxidative enzymes seemd to be beneficial than that of(-)-NBP or(+)NBP.Conclusion The results suggest that NBP improves energy pump and subsides oxidative injury which may contribute to its anti-neuronal apoptotic effect.