AIM To develop less toxic and more effective anti-tumor drugs with the medicinal herb, the various extracts from the herb
Bai-Hua-She-She-Cao(
Hedyotis diffusa ) were prepared and determined of their single and multi action of anti-tumor. METHODS Decoction, lipophilic extract and crude
polysaccharide were prepared from Hedyotis diffusa , with water extraction, alcohol extraction and defatting/water extraction and alcohol precipitation, respectively. Anti-tumor activities were tested with tumor-model in vivo (Sarcoma-180 cells implanted in mice). RESULTS Experimental results indicated that the elementary aqueous extract of H. diffusa had no marked anti-tumor effect on Sarcoma-180 cells implanted in mice. The water-soluble crude polysaccharides separated from H. diffusa and the lipophilic extract of H. diffusa showed the anti-tumor activities,and showed the protective effect on chemotherapeutic damage. Combined administration of the lipophilic extract and the crude polysaccharides did not show the synergistic anti-tumor activity. CONCLUSION The experimental results indicated that the herb will have prospect of application to anti-tumor; the separated extraction is better than simple decoction for the treatment; the further studies on the multi-use of the herbal constituents will be needed.