AIM To study the pharmaceutical characterization and pharmacokinetics of
long circulating liposomes containing daunorubicin.
METHODS The morphology of daunorubicin long circulating liposome was surveyed under the transmission electron microscope. The size of daunorubicin
long circulating liposomes was determined by laser scatter method. The entrapment efficiency and accelerative experiment stability of the daunorubicin long circulating liposomes were examined. Visible spectrophotometry and the HPLC method were established for determination of the daunorubicin in the long circulating liposomes. The percent release of daunorubicin from long circulating liposomes in HBS (pH 7 5) and rat serum at 37℃ were examined. The pharmacokinetics in rats were studied. RESULTS The high entrapment efficiency (>85%) and stabilized long circulating liposomes could be achieved. The drug was slowly released from the daunorubicin long circulating liposomes. The drug released from liposomes was less than 10% in 24 h. The T 1/2 α and AUC of long circulating liposome were higher than those in injections. CONCLUSION The long circulating liposomes prepared by us have high
encapsulation efficiency and the pharmaceutical characterization showed good stability, they can be used for clinical purpose.