AIM To provide molecular evidence for quality evaluation and GAP production of Pogostemon
cablin (Blanco) Benth. cultivated in different regions in Guangdong and Hainan provinces, China, by comparing two
sequences (1 2 kb of plastid mat K gene and 1 8 kb of nuclear 18S rRNA gene) and two
chemotypes (Pogostone type and Patchouliol type in essential oil composition). METHODS PCR direct sequencing was applied to detemine the mat K and 18S rRNA sequences for six samples of Pogostemon cablin from different localities. RESULTS The mat K sequences of six samples of Pogostemon cablin from different regions of cultivation are 1 245 bp in length, which coding 415 amino acids of protein (maturase), and 18S rRNA sequences are 1 803～1 805 bp in size. Based on multiple
Sequence alignment, there are 47 variable sites in the mat K sequence of these six samples, 17 in the 18S rRNA sequence. The cluster tree reconstructed by UPGMA method shows that the sequence divergence both in mat K and 18S rRNA genes among six samples of Pogostemon cablin was well correlative with their regions of cultivation and intraspecific chemotypes of essential oil composition. CONCLUSION Combining with chemical and biogeographical data,
DNA sequencing can become a powerful tool in the key technique
species identification of quality evaluation and GAP production of Pogostemon cablin .
More abstracts about the DNA PROFILING OF POGOSTEMON CABLIN CHEMOTYPES DIFFERING IN ESSENTIAL OIL COMPOSITION