AIM To observe the stability of BCG induced
insulin resistance model. METHODS The glucose tolerance, serum glucose, FFA,
insulin, triglycerides, cholesterol, TNF α and ALT level were measured. The change of GDR was measured by
euglycemic clamp in model rats after given iv BCG 2, 4 and 8 weeks. RESULTS After 2, 4 and 8 weeks, the GIR and glucose tolerance of the animals deceased significantly. After 2, 4 and 8 weeks, BCG infusion resulted in a pronounced reduction in glucose tolerance and insulin stimulated glucose disposal rate
. BCG infusion resulted in a pronounced increase in the weights of the liver <(6 2±0 9) vs (8 2±1 3) g, P <0 05> and spleens <(0 51±0 11) vs (1 4±0 4) g, P <0 01>. The histo pathological results showed that BCG infusion resulted severe inflammation in the livers and spleens and the ratio of β/α in pancreas increased. The serum levels of triglyceride, FFA and glucose were unchanged, but the level of serum TNF α <(543±60) vs (759±137) pg·mL -1 , P <0 05> and insulin <(31±5) vs (36±5) mu·L -1 , P >0 05> increased. CONCLUSION This novel model of immune insulin resistance is completely and constantly established.