Objective To explore the relationship between activated T
lymphocytes (CD25) and
expression of IL 5 mRNA in
sensitized mice, and the effects of triptolide (TP). Methods The peritoneal injection and inhalation of ovalbumin (OVA) were used to establish the sensitized BALB/c mouse model.Immunocytochemistry and in situ hybridization (ICC/ISH) were used in combination to explore the relation between CD25 and expression of IL 5 mRNA in T lymphocytes, and to compare the effects of TP and dexamethasone (DM). Results CD25 and IL 5 mRNA were expressed more intensely in T lymphocytes in sensitized
mice than those in normal controls ( P <0.01): CD25 + /IL 5 mRNA + expression in T lymphocytes in sensitized mice was higher than that in normal controls ( P <0.01): CD25 - /IL 5 mRNA - expression in T lymphocytes in sensitized mice was lower than that in normal controls ( P <0.01): CD25 + /IL 5 mRNA - expression and CD25 - /IL 5 mRNA + expression in T lymphocytes in sensitized mice had those in normal controls( P <0.05).CD25 expression and IL 5 mRNA expression in T lymphocytes were significantly lowered beause of the treatment with TP
groups or DM: CD25 + /IL 5 mRNA + expression in T lymphocytes in TP groups or DM groups was lower than that in sensitized groups ( P <0.01); CD25 - /IL 5 mRNA - expression in T lymphocytes in TP groups or DM groups was higher than that in sensitized groups ( P <0.01); CD25 + /IL 5 mRNA - and CD25 - /IL 5 mRNA + expressions in T lymphocytes in TP or DM groups showed sensitized groups( P <0.05). However, there was no signiticant difference in CD25 + IL 5 mRNA + expression, CD25 + /IL 5 mRNA - expression ,and CD25 + /IL 5 mRNA - expression and CD25 -/IL 5 mRNA + expression between TP and DM treatment. Conclusion Expression of IL 5 mRNA is highly related to the
activation of T lymphocytes. Inhibition of T lymphocytes activation by TP or DM may be one of the mechanisms by which they inhibits the production of IL 5.
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