Objective: The effects of polypeptides extracted from Chlamys farreri (PCF, Mr: 800-1000) on the structure of skin and the
content of antioxidants in the skin of the hairless mice
irradiated by
Ultraviolet B', 1, 1603860)"; href="/tags/ultraviolet-b/">ultraviolet B were studied. Methods: The oxidative damage models of hairless mice irradiated by ultraviolet B (radiating intension: UVB 5.15×10 -2J/cm 2 ×30 d) were established. The mice were randomly divided into five groups: unradiated control and UVB-radiated (distilled water, 5%PCF, 20%PCF and 10%VitC) groups. The number of
fibroblasts was counted and the thickness of
epidermis was measured under microscope, and the ultrastructure of skin was observed under electromicroscope. The content of malonyldialdehyde (MDA), the activities of GSH-Px, SOD and the T-AOC in the supernate of skin homogenate of mice were detected by biochemical methods. Results:The large dilated vacuolations and few rough endoplasm reticulum in fibroblasts and epidermic cells of mice in UVB model group were observed under electromicroscope. PCF could enhance the thickness of epidermis and the amounts of fibroblasts in skin of mice and keep the ultrastructure of epidermis and fibroblasts in the mice normal. Moreover the rough endoplasm reticulum was much more in the fibroblasts of the mice in PCF group than that in UVB group. PCF also increased the content of SOD and T-AOC and decreased the amounts of MDA in the skin irradiated by ultraviolet B. Conclusion: PCF has the protective effect on the hairless mice irradiated by ultraviolet B, which may be mediated by increasing the content of antioxidase and eliminating the free radical.
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