AIM:To explore the pathogenesis of hypoxic
ischemic encephalopathy and functional mechanism of resolving phlem and eliminating
statis by detecting the influence of pujin
koufuye on nitric oxido synthase(NOS) in the cerebral tissue of rats with hypoxic
ischemic encephalopathy.METHODS:Hypoxia ischemia rat models were made in 7 day old SD rats by using the occlusion of right common carotid artery and hypoxia, then pujin koufuye was injected into stomach at 0,2,4,6,8 and 10 hours.The dosages were 2.5 g/kg per day in the high dosage group and 1.25g/kg per day in the low dosage group respectively.The sham operation group and the model group were administrated with normal saline after operation. Tanakan group was filled with tanakan mixed liquid (40 mg/kg per day). After 24 hours,the rats were killed to take out brain tissue in right tip pillow part so as to make 100 g/L homogenate equally plasma,and then the NOS content was mensurated in colorimetry by visible light dispart light photometer.RESULTS:The levels of NOS in high dosage group were(37.60±5.47)μkat/L,which had extremely significant difference from that of model group<(49.97±5.53)μkat/L> (F=0.14,P< 0.01);The levels of NOS in high dosage group was different from that of tanakan group<(42.92±2.70)μkat/L> (F=10.22,P< 0.05). CONCLUSION:Pujin koufuye in the high dosage group can decrease obviously the level of NOS in the cerebral tissue of rats with hypoxic ischemic encephalopathy,the effect of which is better than that of tanakan group.