Aim:By genechip cDNA microarray, the biomolecular mechanism of anti-fatigue of
Pueraria Flavonid was studiedin the center
nerve system(CNS) of the exercise-training rats. Methods: SD rats were randomly divided into three groups: normalrest group(A), exercise-training group(B) and administered-drug group(C). B group and C group rats were trained with highintensity 7 week by treadmill. The total RNAs were isolated from the brain tissue, and were purified to mRNAs by Oligotex.The purified mRNA were reversely transcribed to cDNA with hybridization probes labeled by fluorescent-dUTP. The genechipwas scanned for the fluorescent signals and showed differences by the computer analysis. Results: The GAT-2 gene was up-
regulated expression in the brain of exercise-training rats compared with of rest rats and obviously up-regulated expression in thebrain of administered-drug rats compared with of exercise-training rat. Conclusion: GAT-2 gene expression in CNS of thetraining rats is regulated by
Pueraria Flavonid, and its expression has an effect on CNS fatigue and plays an important role in thestudy of CNS fatigue mechanism and screening anti-fatigue functional food as new predisposing gene.