OBJECTIVE To establish a high performance liquid chromatography (HPLC) method to determine plasma nimodipine concentrations in severe craniocerebral trauma patients. METHODS The plasma samples, added diazepam as the internal standard, were deposited down the protein with methanol. The supernatant was evaporated by N2 at 40℃. The remains were dissolved with 100 μL mobile phase solution; 20 μL of them was injected to the sample; Nova-Pak C18 (3. 9 mm×150 mm, 4 μm) column was used (mobile phase: MEOH: H2O=48:52; flow rate
1. 0 mL·min-1). Ultraviolet detection wavelength was 238 nm, RESULTS The chromatography was good and not interferred by the components of the plasma. The linear range was 2. 5-250 μg·L-1. The lowest detectable limit was
2. 5 μg·L-1. The average recovery was 100. 2%. RSD values of within-day and between-day was not over 10%. Eight patients' samples were determined with this method after intra-venous drip of nimodipine. CONCLUSION This study provides a simple, accurate, reliable method for clinical pharmacokinetic studies as well as determining plasma nimodipine concentration in severe craniocerebral trauma patients.