Objective>To investigate the effect of the“Kechuan Fang”on
eosinophils apoptosis of lung tissue in
guinea-pig with asthma.
Guinea pigs were randomly divided into four groups,asthma model group,prednisone group,“Kechuang Fang”group and normal control group.Eosinophils were isolated from the bronchovesicular lavage.Eosinophils and eosinophils apoptosis counts in every group were measured with biochemistry and immunohistochemistry.Fas-mRNA and bcl2-mRNA expression were determined by means of in situ blotting.The results were analyzed using colour- pathoˉlogical image analysis system.The eosinophils counts and infiltration in“Kechuang Fang”group were significantly lower than those in both asthma model group and prednisone group,but eosinophils apoptosis counts were higher signifiˉcantly than those in that two groups.Eosinophils Fas-mRNA expression of lung in the asthma guinea-pig in“Kechuang Fang”group increased more significant than that of others,but eosinophils bcl2-mRNA expression decreased more signifiˉcant than that of both the asthma model group and prednisone group. The“Kechuang Fang”can reduce eosinophils infiltration in asthma guinea-pig lung tissue,promote eosinophils apoptosis-gene Fas expression,and induce eosinophils apoptosis.