OBJECTIVE To prepare uranine
cationic liposome and evaluate the
transfection efficiency on Staphylococcus Aureus. METHODS
The liposomes were prepared by dehydration-rehydration, sonication, extrusion respectively and then lyophilized. The entrapment efficiency of uranine in the
cationic liposome was detected and the transfection efficiency of the liposome on Staphylococcus Aureus was evaluated by FASC. RESULTS The results revealed that cationic liposome was regular in its morphology and the diameter range of the liposome was 20-50 nm. The mean entrapment efficiency of uranine in the liposome of three preparations was 19.42%. The release properties of the encapsulated uranine from liposome could be expressed by the following equation: Q=2.8293 + 2.7092T( r =0.9839). The results of FASC indicated that 30.1%±12.5% of
Staphyloccus Aureus had integrated the encapsulated uranine.CONCLUSION The cationic liposome could be an idea vector for delivery of encapsulated drugs into Staphyloccus Aureus .