Aim To investigate the pharmacokinetics of magnolol and honokiol in Wistar rat tissue and body fluid.Methods The magnolol and honokiol were simultaneous determined within 15 minutes by a RP-HPLC method on the column C 18 ,a mobile phase consisted of methanol-water-acetic acid glacial(70∶30∶1) with 1ml/min,a detector 294nm with sensitivity 0.005 Aufs.Results There was good linear relationship between both the two drugs' concentration in serum and urine and chromatogram peak area.Two drugs were found to conform to a two compartment open-model with C max 0.974mg·L -1 and 0.522mg·L -1 ,T 1/2β 3.136h and 3.284h,T 1/2ka 0.160h and 0.261h.The drugs were found in many tissues after a single oral dose and the top-level tissue were stomach and intestine.The distributions of the drugs were most in liver,lung and kidney.68.54% of magnolol and 53.81% of honokiol in plasma were shown to be bound to protein.The two drugs were no good assimilation since the total excretion of the parent compound in faces were more than 50% of the dose,and in urine and bile less than 5%.Conclusion Magnolol and honokiol were not up to absorption,metabolized in liver and drained from kidney first.