Objective:To study the cellular and molecular mechanism of Shenshuning(SSN) in preventing
mesangial sclerosis.Methods:Mesangial
cells(MC) stimulated with angiotensin(ATⅡ) were cultured with different concentration of SSN pharmacological serum(2.5%,5%,10%)and with different duration(4 h,8 h,16 h and 32 h).MC proliferation,
secretion of colⅣ or FN and mRNA
expression were measured by MTT, ELISA and RT-PCR.Results:MC proliferation was inhibited by SSN pharmacological serum in a dose-dependent manner under normal and pathological condition. After stimulation of Angiotensin Ⅱ,the secretion of colⅣ and FN reached its peak at 16 h,and began to decline at 32 h. SSN inhi bited the secretion of ColⅣ and FN in a dose-dependent manner. SSN also inhibited the expression of ColⅣ and FN mRNA.Conclusion:SSN is an effective recipe in treating MsPGN.SSN can significantly inhibit the secretion of ColⅣ or FN and its mRNA expression in vitro, which may be the mechanism of SSN in prevention and treatment of glomerusclerosis in MsPGN.