Objective:To observe
pharmacokinetics and
tissue distribution of buflomedil hy-drochloride in rabbits and rats after iv injection
by high performance liquid chromatographic method.Meth ods:The drug in serum or in tissue was extracted into the organic solvent under basic condition.The extracts were analyzed subsequently on a column packed with silica which contained camphorsul-phonic acid in methanol as mobile phase,the detection wavelength was275nm.Re sults:The detected data of drug in serum were analyzed by PK-GPAPH program and the main pharmacokinetic parameters were:K 12 (h -1 )=1.810±0.273,K 21 (h -1 )=0.802±0.184,K 10 (h -1 )=0.431±0.045,Vc(L/kg)=1.250±0.553,T 1/2α (h)=0.260±0.029,T 1/2β (h)=6.017±0.681.The highest concentration in tissue appeared in spleen and kidney after a single iv of10mg ·kg -1 in rats.Conclu sion:The concentration-time curve in rabbits is in accord with2-compartment open model.
Tissue distribution studies showes extensive uptake in organs.