The effects of Ligustrazine and shear stress on rat cerebral microvascular
endothelial cell (rCMEC) were investigated by
administration of the drug incorporated with different levels of shear stress generated by rotational cone-plate rheometer. The influence of Ligustrazine and shear stress on phosphatidylser-ine (PS)
transferring were determined by immunofluorescence of Annexin V -FITC with total internal reflection evanescent wave exciting and ICCD imaging. Results indicated that PS transferring is a popular phenomenon for the membrane of cultured endothelial cells. However, the percentage degree of PS transferring of the non-treated cells in static is 9.97%. As a single factor, shear stress of 6×10-5, 12×10-5, 24×10-5 N/cm2 or Ligustrazine of 31.5, 63.0, 126.0 ×,g/ml reduced PS transferring significantly (P<0.001). The rate for the cells treated by Ligustrazine is lower than that of non-treated cells at the same level of shear stress. Moreover, the middle-dose
combination of the two factors appears to be comparatively effective for lowering the rate. The results indicate that apoptosis of rCMEC might be restrained by a combination of certain level of shear stress with a suitable dose of Ligustrazine.