In order to study the
protective effects of trifluoperazine (TFP) against hypoxic brain injury, the effects
of TFP on population spike (PS) of slices during hypoxia and the changes of content of amino acids in the culture medium of brain slices were studied by the extracellular recording tech-nique in rat
hippocampal slices and the HPLC fluoromatric method. It was showed that in the hippocam-pal slices treated with TFP of 50 μ mol/L, the mean disappearance time of PS after hypoxia was signifi-cantly higher than that of oxygen and glucose deprivation (OGD) group(P<0.05), and the mean recovery amplitude of PS after reoxygenation was 52.8% ± 14.3%,significantly higher than 20.5% ± 9.8% of that of OGD group (P<0.01). TFP can also markedly inhibit the increase of the excitatory amino acid (EAAS) in the culture medium of brain slices during hypoxia (P<0.05). Therefore, TFP has a
protective action against brain hypoxic injury. The mechanism may be that TFP can inhibit exciting amino acids (EAAS) release from hippocampal tissue.