AIM:To establish a reversed-phase HPLC
method with fluorescence detection for determination of the concentration of
propofol in human plasma. METHODS: Agilent 1100 HPLC instrument was used with the column : Diamonsil C 18(150 mm×4.6 mm,5 μm), Phenomenex Security Guard column(4 mm×3 mm).
plasma samples were precipitated with the thymol (internal standard) methanol solution. 20 μL of supernatant fluid was injected for HPLC analysis. The mobile phase was composed of acetonitrile-water(56:44,V/V).The flow rate was 1.5 mL·min -1. The fluorescence detective wavelengths were: λ ex=276 nm, λ em=312 nm. RESULTS: The
standard curve equation was Y= 1.787C- 0.031 (r= 0.9997,n=9).The linear range was (0.02—8.0) mg·L -1. The average recovery was 98.71 %.Intra- and inter-day precision(RSD%) were all less than 4.2 %. The limit of detection was 4 μg·L -1(S/N=2). CONCLUSION:The RP-HPLC method is simple, sensitive and accurate for monitoring propofol concentration in clinical plasma samples.
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