AIM To explore mechanism of PLC inhibition on platelet adhesion by quantitive determination of PLC influence on human platelet
membrane GPIb quantity (ng) with competitive enzyme-linked immunosorbent assay (CELISA). METHODS
Standard curve are drawn with CELISA kit. Washing platelet are made from healthy human blood and divided into four parts: (1)
physiological saline part;(2)PLC 0. 5 U part; (3)PLC 1. 0 U part; (4)PLC 2. 0 U part to replace standard IgGCIgG-5T). Then OD492 are tested with TECAN. The experiment was made for 6 times. GPIb quantity(ng) was calculated according to OD492 by standard curve and Eocel. Their correlation are tested with t value. RESULTS OD492 of physiological saline, PLC 0. 5 U, 1.0 U, 2. 0 U were 0.768 ± 0.078, 1.103±0.018, 1.367 ± 0.102, 2.055 ± 0.453 respectively, and their corresponding GPIb quantities (ng) are 134.669 ± 13.144, 101.838 ± 9.879, 82.632 ± 5.354, 73. 183?±14. 74 respectively. The differences of GPIb quantities are significant a-mong PLC 1. 0 U , PLC 2. 0 U and physiological saline ( P < 0. 01), but not notable among other parts(P>0. 05). CONCLUSION PLC can reduce platelet membrane GPIb quantity apparently, so it can inhibit platelet adhesion. It may be one of the important reasons of PLC anti-aggregation on platelet.