Objective:To develop a HPLC method for the determination of 17997 in plasma. Method: Samples of plasma (250μL)were extracted
with 500μL acetonitrile, and then centrifuged at 12 000 r · min-1 for 3 min at 4 ℃. Injected the supernatant 50μL into the chromatography directly. 17997 were analyzed on Waters μ- Bondapak C18 (3. 9 mm×300 mm, 10μm) analytic column connected with μ- Bondapak C18 (Guard - PakTM Cartridge )prec-olumn at 40℃ with a methanol - double distilled water(75: 25) mobile phase and detected at 304 nm. The flow rate was 1 mL ·min-1. Results: 17997 was eluted 7. 0 min, after injection. Calibration plots in plasma were linear(r = 0. 999 7 , n = 5 ) from 0. 05 to 5μg· mL . For 5,1,0. 1μg·mL check samples, intra - run ( n = 7 ) precisions (RSD) were 1. 7% -3.5% and inter - run( n =5 )precisions( RSD) were 0. 64% -6. 6 %,
respectively. The average relative recoveries were( 101 ± 0. 6 ) % , ( 101 ± 3. 7 ) % , ( 104 ± 6. 8 ) % for the high, middle and low check samples,respectively. The limit of detection was 0. 01μg·mL . Conclusion:The developed analytical method for 17997 was found to be sensitive, simple and rapid, suitable for application in preclinical and clinical
pharmacokinetic studies.