Aim: To investigate the inhibiting role of
Salvia miltiorrhiza monomer IH764 3 in proliferation and collagen synthesis and
the effect on the expression of focal adhesion kinase in
hepatic stellate cells( HSCs) stimulated by H 2O 2 in vitro, so as to provide evidences for preventing hepatic fibrosis. Methods: The effects of IH764 3 on the proliferation and collagen synthesis of HSCs were examined by 3 H TdR and 3 H proline incorporation assay, respectively. The expression of FAK mRNA was examined by RT PCR in lysates of cultured HSCs. Results: The proliferation and collagen synthesis were inhibited in dose dependent and time dependent manners. The expressions of FAK mRNA in the IH764 3 (10 μg/ml, 20 μg/ml, 30 μg/ml, 40 μg/ml) groups were significantly lower than that in the control group. Conclusion: The present results demonstrate that IH764 3 can inhibit HSC proliferation and collagen synthesis. Meanwhile the mRNA transcription of FAK is reduced significantly, FAK as a signal molecule, can be involved in the effect of IH764 3. This may be one of the anti fibrotic mechanisms of Salvia Miltiorrhiza.