OBJECTIVE To investigate the
enantioseparation of
methocarbamol using
capillary electrophoresis(CE) with three
cyclodextrins as the
chiral additives.METHODS The enantioseparation of methocarbamol was processed with β-
Cyclodextrin(β-CD),dimethyl-β-cyclodextrin(DM-β-CD) and 2,6-di-O-carboxymethyl-β-cyclodextrin(CM-β-CD) as the chiral selectors.The effects of the pH,composition and concentration of the
running buffer solution,the sort and concentration of chiral selectors,the running voltage,the capillary temperature on the mobility and chiral separation were studied respectively.In addition, the mechanism on chiral distinguish was discussed.RESULTS CM-β-CD gave a baseline enantiomeric separation for the basic drugs,whereas the β-CD and DM-β-CD,under optimal conditions,showed poor stereoselectivity and gave just partial chiral separation.CONCLUSION Lower pH of the running buffer solution tends to be more favorable for the enantioseparation of the basic drugs with acidic derivatized cyclodextrins.
More abstracts about the Enantioseparation of methocarbamol by capillary zone electrophoresis using cyclodextrins as chiral a