Objective: To develop a new method for det er mination of Mangiferin in the leafs of Folium
mangiferae. By this new method
, Mangiferin in F.
mangiferae sampled in different months and in different r egions was determinated. Method: A RP-HPLC method was set up, using Shim pack CLC-ODS column, methanol-0.05 mol·L -1 H 3PO 4 (65 ∶134, pH 3.5) as mobile phase, with 258 nm of detection wave, at room tem perature, 1 mL·min -1 . F. mangiferae sampled in Nanning, Qinzhou an d Tianyang, Guangxi province and sampled respectively in January to December we re determinated. Result: The average recovery of the RP-H PLC was 99.2%, RSD=1.05%(n=5). The
content of Mangiferin in F. mangifera e was statistically different due to different sample-regions or sample-time . Conclusion: This RP-HPLC method is simple, specific and exact. The contents of Mangiferin in the leafs of F. mangiferae sample in Nanning and Tianyang were statistically similar, but higher than that in Qinzho u. The contents of Mangiferin in the leafs of F. mangiferae sampled in July to October were higher than that in the other months. The content in September was the highest, the content in February was the lowest.