Objective: To observe the effects of silym arin on hepatic microsomal and mitochondrial membrane fluidity in mice. Method:
Live r
microsomal and mitoc hondrial membranes were labled by ANS and DPH. Membrane fluorensent intensity ( F ), fluorensent polarization(P) and microviscosily(η) of liver microsome and mitoc hondria were determined. Result: Sil increased the externa l membrane fluidities of liver microsome and
mitochondria, and decreased the internal membrane fluidit ies of liver microsome and mitochondria. Pretreatment with CCl 4, the external me mbrane fluidity of liver microsome and mitochondria were increased, and the inte rnal membrane fluidities of liver microsome and mitochondria were decreased. Aft er given sil 140,280 mg·kg -1 , the increased external membrane fluidities of live r microsome and mitochondria were lowered , and the decreased internal membrane fluidities of liver microsome and mitochondria were enchanced in a dose-depende n t manner. Conclusion: The protective effects of sil on liv er injury may be relat ed to the recovery of the membrane fluidities of liver microsome and mitochondri a.