To explore the mechanism of Jianpi(splenic tonic) and Shugan(dispersing the stagnated liver-qi) prescription regulating immunity by testing the expression of the differentiation signaling transduction protein,calcineurin.Methods We prepared the Sijunzitang and Chaihushugansan prescription serum,take the Ova as antigen induction and B cell as presenting cell,intervened culturing the CD 4 +T cells for 24 hours, and then examine the expression of calcineurin by RT-PCR,Western-blotting.Results The expression of calcineurin was increased in the stimulated Th cells, and the Sijunzitang and Chaihushugansan prescription serum can reduce it significantly,the Chaihushugansan also improved the expression deficit of calcineurin.Conclusions Jianpi and Shugan prescription can regulate the balance of immunity through affecting the expression of signaling transduction proteins at the Th activation and differentiation stage,The regulating the flow of qi prescription is superiority to supplementing qi one.in treatment of the Th2-mediated autoimmune disease.