Objective:To study the protecting effect of delayed
preconditioning with A 1 selective adenosine receptor agonist in donor
heart preservation. Methods:Wistar rats were injected with 2 chloro N6 cyclopen tyladenosine (CCPA). 24 hours later,the hearts were isolated and mounted on a Langendorff apparatus,perfused with filtered modified Krebs Henseleit buffer through aorta. The hearts then were arrested with 4 ℃ modified St.Thomas solution and stored at 4 ℃ for 4 hours ,and reperfused with K H buffer for one hour. Left ventricular function,myocardial CK mb leakage and tissue levels of adenosine triphosphate(ATP) were measured. Results:The maximum recovery rates of increase and decrease in the left ventricular presure( ±dp/dt max ,%) were 62.83±17.27 and 66.81±18.99 respectively in the preconditioned group, compared to 40.41±18.29 and 44.70±25.14 respectively in the control group. The ATP level (10 -3 μmol/wet.g) was 3.67±1.42 in the preconditioned group, compared to 1.46±0.54 in the control group.The difference were significant ( P <0.05). Conclusion:This study suggests that the transient
preconditioning with CCPA may have some beneficial effects on Wistar rat
donor heart preservation.