OBJECTIVE:The methodology of
quantitative analysis of icariin in Chinese herb
Heiba Epimebii was investigated and the content
of ICARIINE OF
HEIBA EPIMEBII in different parts,different variety and different planting areas of the plant was also measured.METHOD:The 23 samples in seven variety of Heiba Epimebii were measured with RP HPLC technique.The analysis column of this method was PERKIN EIMER SH/5C 18 column, the flow phase was CH 3CN water 36% acetic acid(25∶73.5∶1.5) and flow velocity was 1.0ml/min.The wavelength of UV for the analysis was 270nm.RESULTS:There are excellent linear correlation in two ranges,0.0212～0.127μg and 0.53～1.696μg,with this
quantitative method.The content of icariin in different parts,different variety and different planting areas of the plant were 0.00307%～1.55%.The quantitative distribution of icariin in a plant of Heiba Epimebii is leaf>leaf stalk>stem>root.CONCLUSION:The content of icariin was obviously variable in different variety and different planting areas of Heiba Epimebii.This quantitative measurement method is an easy way to select good variety and extend source of this kind of Chinese medicine