Objective:To establish a high performance capillary electrophoresis ( HPCE ) method for the separation of active ingredients
in kitasamycin. Methods:A study of the separation of active ingredients in kitasamycin by
microemulsion electrokinetic chromatography ( MEEKC ) is presented. The effects of some factors in the electrophoretic conditions on the separation were investigated Results: The satisfactory separation was achieved on optimization of electrophoretic condition: 0 08 mol·L -1 heptane-10% isopropanol - 0 01 mol·L -1 sodium tetraborate buffer - 0 12 mol·L -1 SDS (pH 9 0) as microemulsion with on - column UV monitoring at 214 nm,25 kV applied voltage, 30 ℃ column temperature The relative standard deviations of migration time and peak area of A 5 for replicate injections ( n =6) were 0 7% and 1 9% ,respectively Conclusion:The results demonstrated that MEEKC would be a promising technique for separation and determination of strongly hydrophobic neutral compounds with similar structure