Objective:To determine the contents of
anthraquinone before and after hydrolysis in the
commercial medicinal materials of
Radix Polygoni Multiflori and Radix Polygoni Multiflori Preparata collected from different producing areas respectively by RP-HPLC.Method:This method used a column of Inertsil ODS-3 and methanol-0 1% phosphoric acid solution(85∶15)as the mobile phase,the detection wavelength was 254 nm.Results:The method recoveries of emodin,physcion,rhein,chrysophanol and aloe-emodin before hydrolysis were 98 1%,98 8%,99 2%,99 9%,99 8% respectively with corresponding RSD of 0 8%,2 1%,0 9%,1 9%,1 5%(n=5) and after hydrolysis were 96 2%,96 1%,98 3%,97 6%,97 4% respectively with corresponding RSD of 0 7%,0 6%,1 8%,1 6%,2 2%(n=5).Conclusion:
Anthraquinone contained in the samples were mainly emodin and physcion ,besides,trace rhein,chrysophanol and aloe-emodin were detected in some samples.The contents varied greatly in the samples from the different producing areas.