A
capillary electrophoretic method was established for the separation and determination of
omeprazole in human
plasma with theophylline as the internal standard and dichloromethane as the extracting solvent.Based on the mode of capillary zone electrophoresis,electrophoretic separation was achieved with 50 mmol/L phosphate 12.5 mmol/L borate (pH 10.10) running buffer and a constant voltage of 16 kV applied to the CElect FS50 uncoated capillary(45 cm×50 μm).The analytes were introduced into capillary by pressure(15 Psi×s) and determined with on column UV monitoring at 200 nm.No interference was found in the omeprazole or internal standard theophylline peak situation.The good linear relationship was obtained within the range of omeprazole plasma concentration 100.0～5000.0 ng/ml( r=0.9991,n=5 ).The method recovery was more than 95% and the intraday or interday precision in replicate injections( n=5 ) was less than 9.0%.This capillary electrophoretic method has been successfully applied to clinical plasma samples analysis and the pharmacokinetic study after a single infusion administration of 80 mg omeprazole to 4 healthy volunteers.
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