OBJECTIVE:To develop a reversed phase HPLC method for the
quantitative determination of the
ergot fermentation product α
ergocryptine and to determine the peak value of α ergocryptine yielded during the fermentation.METHODS:A YWG C 18 column (250 mm×4.6 mm ID,particle size,10 μm) was used;the mobile phase was MeOH phosphate buffer (pH7.0) EtOAc (75∶25∶1) with a flow rate of 1.0 ml·min -1 and detected at 312 nm.A
regression equation was set up,the precision of the method and the recovery rates were measured.RESULTS:Regression equation was A=19058.54M+1352.66,r=0.9997,over the range of 0.6375 ～6.4375 μg;the RSD for the intra day and inter day was 1.15%,0.44%,respectively;the mean recoveries were 108.48%,105.15% and 109.53% versus the added authentic sample at the concentrations of 122,244 and 488 μg·ml -1 ,respectively.The peak value appeared between day 15 to day 17 under the given condition.CONCLUSION:The method is sensitive and reproducible for the quantitative determination of the ergot fermentation product α ergocryptine.
More abstracts about the RP-HPLC assay for the determination of α-ergocryptine in the ergot fermentation products