OBJECTIVE To study the protective effects of
piperoethyl acetyl amine (PAA) on hepatotoxin induced liver injury in mice.METHODS
Those mice were treated with a dose of PAA 1 h before or two doses of PAA 24 h before in injection of CCl 4 or
D galactosamine.Serum transaminase (ALT) and liver glycogen were determined.The damage of liver tissues was observed by light microscope.Malondialdehyde (MDA) production of liver microsomes initiated by CCl 4 in vitro was measured.RESULTS Administration of PAA prior to CCl 4 significantly prevented the elevation of serum ALT and the decrease of liver glycogen in CCl 4 or D galactosamine intoxicated mice.The damage of liver tissues was also reduced.CCl 4 initiated lipid peroxidation (MDA formation) of liver microsomes in vitro was inhibited by PAA in a dose dependent manner.CONCLUSION The hepatoprotective action of PAA against CCl 4 and D galactosamine intoxication in mice may be related to its inhibitory effect on CCl 4 initiated microsomal lipid peroxidation and the increase of liver glycogen.