Objective:To examine the variation of alcohol extraction rates of Ganoderma lucidum spores with different rates of wall-wrack,and
analyze the
antitumor components of alcohol extract by chromatography.Method:The G. lucidum spores were soaked and extracted with absolute alcohol. The alcohol extract was chromatographed on a silica gel column and HPLC in proper order,and the
antitumor activity of every eluted fraction was represented by its cytotoxicity towards Hela cells.Result:Extraction rates 5%, 25% and 33% corresponded to wall wrack rates 0%, 60%～80% and 99% respectively.The alcohol extract from spores with the highest wall-wrack rate was chromatographed on a silica gel column, eluting successfully with CHCl 3, EtOAc and CH 3OH in order. The CHCl 3 fraction had not any antitumor activity,while this activity of CH 3OH fraction was 34 times greater than that of EtOAc fraction. HPLC analysis found out that two mixtures(Ⅱ 1 and Ⅱ 3) possess significant antitumor activity in vitro.Conclusion:The weight of alcohol extract from spores with wall wrack was far greater than that of spores without.The antitumor components of G. lucidum spores could be analyzed with methanol-water on a reverse HPLC.