Objective:Search new polymerizing agent of bovine hemoglobin for the use of prepare suitable
blood substitute.Methods:Find
new polymerizing agent with the theories of kinetics and thermodynamics of polymerization reaction,compare the characteristics among polymerizing reactions and products of
2 hydroxyl propionaldehyde,glycolaldehyde and glutaraldehyde with electrophoresis and HPLC.Use computer technology to compare and simulate the structures between 2 hydroxyl propionaldehyde and glycolaldehyde polymerized products.Results:The reaction of 2 hydroxyl propionaldehyde polymerizing bovine Hb was mild,its products were stable and mostly made of lowly polymerized polyHbs,their oxygen affinity was suitable to supply oxygen to tissues.The distribution of the products’ molecular weight was much more better than glycolaldehyde polymerized products,and this supplied the possibility that the adverse effects induced by ultra polymerized and unreacted Hbs in the
blood substitute made from glycolaldehyde polymerized bovine Hb could disappear when blood substitutes manufactured with 2 hydroxyl propionaldehyde were used.Conclusion:2 hydroxyl propionaldehyde is a new agent that is very suitable for the manufacture of blood substitutes.\;<