To observe the effect of refined Xuefu capsule (RXFC) on gene expression of myocardical ischemia by in situ hybridization (ISH) and explore the molecular mechanism of acting against myocardical ischemia. There was remarkable
difference on the release of LDH, CK and GOT between rabbit
serum group on the same concentration and medical serum group including RXFC group,XFC group and
ditiazem group(p< 0.01). There had also a remarkable statistical difference between RXFC group, XFC group and ditiazem group on the inhibition of releasing IDH(p < 0.01) . ISH evidence indicated that the
hybrid result of ET - 1 gene was as follows. There was no hybrid granule between RXFC group and normal culture cell group. There were plenty of hybrid granules between ditizem group and anoxic and sugar deficient group. The hybrid result of ECE-1 gene was as follows. There were plenty of hybrid granules in cytosol around myocardium kargon among anoxia and sugar deficient group, rabbit serum group and ditiazem group. There was no significant difference among these three groups. There was no positive hybrid signal among RXFC group, XFC group and normal culture group. It indicated that RXFC possess protective function on myocardium. It was related to inhibiting gene expression of ET - 1 and ECE - 1.