To study the effect of Stichopus Variegatus Compound (ST co.) on the rat's plasmic TXB 2、6-K-PGF 1α 、PGE 2、Fibrinogen (Fg) and platelet aggregation.. In this experiment, ELISA was performed to measure the rats' plasmic TXB 2、 6-K-PGF 1α 、 PGE 2 of each group. In addition, Immune nephelometry assay was imployed to analysis the level of rats' plasmic Fg, in the mean time, Platelet aggregation meter was used in observing the ratio of rats' platelet aggregation at 3 min. and 10 min, respectively. The results showed that the plasmic level of TXB 2、 PGE 2、 Fg of treated group was distinctively lower than that of control group, and the content of 6-K-PGF 1α was higher than that control group (P<0.05), and the ratio of platelet aggregation of treated group was significantly different vs control group (P<0.001). The study suggested that ST co. can significantly reduce the level of rats' plasmic TXB 2、 Fg、 PGE 2 ad elevate the level of 6-K-PGF 1α ; It also plays a role of inhibiting platelet aggregation. ST co. is an effective drug of anti coagulation and thrombosis.