AIM: To establish a reversedphase high performance liquid chromatography (HPLC) method to determine the concentration of
sparfloxacin in patient's plasma. METHODS: The HPLC cloumn was 150 mm×46 mm ID (YWG RPC 18 ,10 μm). The mobile phase was composed of acetate buffer solution (50 mmol/L, pH=50)acetonitrilemethane10% triethylamine (70∶20∶10∶04 V∶V, adjust pH to 240). Detection wavelength was 299 nm. The plasma sample containing phydroxyacetophenone (IS) was extracted singly with 7 ml of methylene chloride. RESULTS: The regression equation of
sparfloxacin with the specific value of peak height Y to the concentration c was Y=2556c-0139 8 (r=0999 1, n=8). The minimum detection limit was 01 μg/ml. The average recovery was 10054%. THe RSD of within day and daytoday were less than 4%. From 36 sex patients, the range of the plasma drug concentrations was between 038 μg/ml and 180 μg/ml at 12 h after taking 200 mg sparfloxacin orally. CONCLUSION: The RPHPLC method is accurate, sensitive, simple, and rapid. It is applicable to determine the concentration of sparfloxacin in human plasma.