Objective:To investigate effect of Baoyuan Paidu Wan on injuries of pig LLC-PK1 ells induced by
cisplatin and the changes of SOD and NO value.Methods:LLC-PK1
cells were divided into four groups for culture.Cells were respectively cultivated in blank
serum group, in serum with Baoyuan Paidu Wan group,in
model group with cisplatin of different concentration and in model group with cisplatin of different concentration plus 10% serum with Baoyuan Paidu Wan. The OD value was measured with MTT method in 12 h and 24 h after the culture. Then the cells livability was calculated according to MTT results. The content of SOD and NO were tested in the cell fluid in 24 h after the culture.Results:Compared to blank serum group, the OD value was increased significantly in serum with Baoyuan Paidu Wan group(P<0.01). The cell livability in model plus serum with Baoyuan Paidu Wan group was obviously higher than that in model group (P<0.01). The content of NO in cell fluid in model plus serum with Baoyuan Paidu Wan group was significantly lower than that in the model group(P<0.01).Conclusion:Baoyuan Paidu Wan could promote the proliferation of LLC-PK1 ells and decrease nephrotoxicity caused by cisplatin, which might be through its anti-oxidation function.
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