AimTo screen monoamine oxidase(MAO)
inhibitory activity from 20 strains of Cordyceps and its allies,and ascertain a strain
from 10 strains of Paecilomyces tenuipes and its active site with higher MAO
inhibitory activity.MethodsThe spectrophotometric and microplate assay for MAO activity based on the consumption of the substrate kynuramine was performed according to Weissbach et al.with some modifications.ResultsOf all species,RCEF1054(Beauveria amorpha),RCEF0943(Paecilomyces cicadae), RCEF1078(Nomuraea atypicola) and RCEF0440(Paecilomyces tenuipes) had stronger MAO inhibitory activity,and their rate were 100%,88.8%,85.3% and 77.2%,respectively.The CHCl_(3) extracts from mycelia of 10 strains of Paecilomycestenuipes had stronger MAO inhibitory activity,the rate of Pt25 had the highest activity(63.66%),6.5 times of Pt02.However,MeOH and water extracts from residue of CHCl_(3) extraction almost had no activity.Extracts frombrothof Paecilomyces tenuipesshowed moderate MAO inhibitory activity,the rates of pt12,pt02 and pt57 were higher than others.ConclusionsMost samples from
different species or strains or extracts by different solvents had different MAO inhibitory activity.Extracts of Paecilomyces tenuipesRCEF0788(pt57) showed higher MAO inhibitory activity.