Objective:To study the protective effects of cuttlebone polysaccharide CPS-1 on experimental ulcerative colitis(UC) in mice.Methods: Dextran sodium sulfate(DSS) was used to induce UC in mice and mice treated with SMP+TMP were taken as control.The protective effects of cuttlebone polysaccharide CPS-1(high and low dose) on experimental UC in mice were evaluated with clinical symptoms,body weight changes and pathological changes in local intestinal mucous membrane.Cytokines related to endothelial cell proliferation and inflammation were determined in mice serum using ELISA kits.Results: There was no obvious change in body weight in mice treated with CPS-1 and the mice were well alive.Serum EGF and PDGF in CPS-1 treated mice were obviously increased compared with those in normal mice,while serum TNF-α in CPS-1 treated mice was significantly lower than that of positive control mice(P<0.05).Conclusion: Cuttlebone polysaccharide CPS-1 can obviously increase EGF and PDGF levels in experimental UC mice and accelerate the concrescence and repair of ulcer.Meanwhile,it can also decrease the expression of TNF-α and relieve inflammation.