Objective To observe the influences of Qingkailing Injection(QI) on apoptosis of(hippocampal) neurons and mitochondrion membrane
potential(MMP) in rat model of hypoxia-(hypoglycemia-)rexyenation(HHR).Methods
Mitochondrion activity was detected by methyl(thiazolyl) tetrazolium test(MTT).The apoptosis percentage and MMP were detected by flow(cytometry),and the cytomorphological changes and neuron necrotic percentage were observed with(fluorescence) microscope.Results After hypoxia-hypoglycemia treating in rats for 5 hours and reoxygenation treating for 3、12、24 hours the apoptosis rate of neurons increased significantly.There were chromatinic coacervation,(karyorrhexis) and apoptotic bodies in neurons were observed cytomorphologically.The MMP and(mitochondrion) activity decreased significantly after reoxygenation for 3、12、24 hours,and decreased(further) along with the prolongation of reoxygenation.QI could decrease obviously percentage of neuron apoptosis and necrosis and increase MMP and mitochondrion activity with a significant difference(P<0.05～0.01) compared with the HHR group.Conclusion Qingkailing Injection could inhibit the decrease of MMP induced by injury of hypoxia-hypoglycemia-reoxygenation,stabilize MMP,inhibit cell apoptosis and protect hippocampal neurons.